
PLoS ONE: Targeted KRAS Mutation Assessment on Patient Tumor ...
Minor grove binder (MGB) modifications at the 3′-end of sequence specific oligos provide the advantage of designing short probes with higher melting temperature (Tm) and increased duplex stability and specificity in comparison to conventional .... As measured with UV-spectrometry, values corresponding to DNA quantity did not differ between groups A and B (Table 1); the samples of the two groups, however, differed substantially in the obtained absorbance values (A260 /280 ...
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